DNA Structure in Sheared and Unsheared Chromatin
- 21 May 1976
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 192 (4241) , 796-798
- https://doi.org/10.1126/science.1265482
Abstract
Shearing chromatin, by either sonication or vortex homogenization, introduces significant structural artifacts. These may be detected by the anomalously large increase in the number of ethidium bromide binding sites and the large alteration of the circular dichroism spectra of chromatin. Structural alterations are also suggested by the disappearance of differential light scattering after shearing.This publication has 25 references indexed in Scilit:
- Circular dichroism and ethidium bromide binding studies of chromatin from WI-38 fibroblasts stimulated to proliferateChemico-Biological Interactions, 1975
- Thermal denaturation of the DNA-ethidium complex. Redistribution of the intercalated dye during meltingBiochemistry, 1975
- A temperature‐sensitive cell cycle mutant of the BHK cell lineJournal of Cellular Physiology, 1974
- Effects of 5-bromo-2′-deoxyuridine and dimethyl sulfoxide on properties and structure of chromatinJournal of Molecular Biology, 1974
- Preparation of ChromatinEuropean Journal of Biochemistry, 1974
- Approach to the structure of native nucleohistoneBiochemistry, 1973
- Protein conformation in biomembranes: Optical rotation and absorption of membrane suspensionsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1972
- Conformation of deoxyribonucleic acid in chromatin: A circular dichroism studyJournal of Molecular Biology, 1970
- Circular dichrosim of calf liver nucleohistoneBiochemistry, 1970
- Correlation of light scattering and absorption flattening effects with distortions in the circular dichroism patterns of mitochondrial membrane fragmentsBiochemical and Biophysical Research Communications, 1969