Acetylene Reduction Assay for Nitrogenase Activity: Gas Chromatographic Determination of Ethylene Per Sample in Less Than One Minute
- 1 May 1980
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 39 (5) , 1078-1080
- https://doi.org/10.1128/aem.39.5.1078-1080.1980
Abstract
Ammoniacal silver nitrate (10 mg/ml) was added to terminate acetylene reduction assays used to measure nitrogenase activity. Silver nitrate quantitatively precipitated acetylene as the carbide salt, but did not affect the ethylene formed. The vials containing ethylene can be analyzed gas chromatographically at the rate of about 13 samples in 10 min.Keywords
This publication has 5 references indexed in Scilit:
- Absence of the Pigments of Photosystem II of Photosynthesis in Heterocysts of a Blue–Green AlgaNature, 1970
- The Acetylene-Ethylene Assay for N2 Fixation: Laboratory and Field EvaluationPlant Physiology, 1968
- In situ studies on N2 fixation using the acetylene reduction technique.Proceedings of the National Academy of Sciences, 1967
- ATP-dependent reduction of azide and HCN by N2-fixing enzymes of Azotobacter vinelandii and Clostridium pasteurianumBiochimica et Biophysica Acta (BBA) - Enzymology, 1967
- Acetylene reduction by nitrogen-fixing preparations from Clostridium pasteurianumBiochimica et Biophysica Acta (BBA) - General Subjects, 1966