Cytokines Enhance the Production of a Chemotactic Factor for Polymorphonuclear Leukocytes by Rat Renal Glomerular Epithelioid Cells

Abstract
Human recombinant interleukin-1.beta. (IL-1) and tumor necrosis factor-.alpha. (TNF) dose-dependently enhanced the production of PMN chemotactic factor, which was trypsin-sensitive and heat-stable, by the epithelioid cells of rat renal glomeruli. Dexamethasone dose-dependently suppressed the chemoattractant production. Molecular weight and isoelectric point of this factor were 10 kDa and over 10, respectively. These results suggest that PMN migration to glomeruli occurs without participation of complement system when TNF or/and IL-1 are produced by activated cells in glomeruli.

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