AMPEROMETRIC MICRO-BIOSENSOR ARRAY FOR CHOLINE AND ACETYLCHOLINE BASED ON A GLASSY CARBON ELECTRODE MODIFIED BY PLATINUM-BLACK PARTICLES AND A CO-CROSSLINKED ENZYME SYSTEM
- 4 February 2001
- journal article
- Published by Taylor & Francis in Analytical Letters
- Vol. 34 (4) , 491-501
- https://doi.org/10.1081/al-100002590
Abstract
A glassy carbon electrode was first modified by electro-depositing sub-micrometer platinum black particles on the surface. An acetylcholine micro-biosensor array and a choline micro-biosensor array were fabricated based on immobilization of acetylcholineasterase-choline oxidase or choline oxidase by cross-linking with glutaraldehyde on the Pt-black/GCE. Significant enhancement in performances of these biosensors was achieved. The chronoamperometric response of the acetylcholine micro-biosensor array gave a linear relation in the range of 29 to 1200μmol/L and a detection limit of 8.7μmol/L acetylcholine. The amperometric response of the choline micro-biosensor array gave a linear relation in the range of 24 to 1000μmol/L and a detection limit of 7.4μmol/L choline. They also possessed very good stability and reproducibility for long-term use.Keywords
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