PRIMING OF DNA POLYMERASE IN NUCLEI OF SEA URCHIN EMBRYOS BY NATIVE DNA
- 1 March 1967
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 57 (3) , 841-848
- https://doi.org/10.1073/pnas.57.3.841
Abstract
Native DNA is a far more effective primer for the DNA polymerase in nuclei obtained from sea urchin embryos than heat-denatured DNA under conditions in which the Mg++ concentration, the amount of primer, the amount of enzyme, and the time of incubation were varied. The consistency of this preference was established with different nuclear preparations and DNA obtained from different sources. The extent of destruction of the ordered helical structure of calf thymus DNA by heat denaturation parallels the decrease in its priming ability.This publication has 17 references indexed in Scilit:
- The Role of Polyamines in the Neutralization of Bacteriophage Deoxyribonucleic AcidPublished by Elsevier ,2021
- Deoxyribonucleic acid polymerase from Walker 256 carcinosarcomaBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1965
- Enzymic synthesis of deoxyribonucleic acidJournal of Molecular Biology, 1964
- Deoxyribonucleic acid polymerase in L cellsBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1964
- DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI1964
- ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID .15. PURIFICATION + PROPERTIES OF POLYMERASE FROM BACILLUS SUBTILIS1964
- STUDIES ON SYNTHESIS DEOXYRIBONUCLEIC ACID BY MAMMALIAN ENZYMES .I. INCORPORATION OF DEOXYRIBONUCLEOSIDE 5]-TRIPHOSPHATES INTO DEOXYRIBONUCLEIC ACID BY PARTIALLY PURIFIED ENZYME FROM REGENERATING RAT LIVER1964
- THE ISOLATION OF NUCLEI FROM EGGS AND EMBRYOS OF THE SEA URCHINThe Journal of cell biology, 1962
- ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID .9. POLYMERASE FORMED AFTER T2 BACTERIOPHAGE INFECTION OF ESCHERICHIA COLI - NEW ENZYME1962
- Thermal Conversion of Nonpriming Deoxyribonucleic Acid to PrimerJournal of Biological Chemistry, 1959