Increase of c‐jun mRNA upon hypo‐osmotic cell swelling of rat hepatoma cells

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Abstract
C‐jun mRNA levels were increased in rat hepatoma cells (H4‐II‐E‐C3) when exposed to hypotonie medium (205 ) with a maximal induction observed after 1 h of hypotonie exposure. At this time point an approximate 5‐fold increase in c‐jun expression could be detected in relation to nonnotonic control incubations (305 ). Hypertonic exposure (405 mosmol/1) had only a slight effect on c‐jun expression. In contrast to the increased c‐jun mRNA levels under hypotonic conditions, expression of the c‐fos proto‐oncogene was unaffected by changes in the osmolarity. The hypotonicity‐induced increase in c‐jun expression was also detectable in the presence of a protein kinase C (PKC) inhibitor. This indicates that PKC is not involved in the signal transduction pathway leading to c‐jun expression upon hypotonic cell swelling in these cells.