Elevated Expression of P-Glycoprotein in Kidney and Urinary Bladder Cancers.

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Abstract
MORIYAMA, M., SUGAWARA, I., HAMADA, H., TSURUO, T., KATO, T., SATO, K., HIKAGE, T., WATANUKI, T. and MORI, S. Elevated Expression of P-Glycoprotein in Kidney and Urinary Bladder Cancers. Tohoku J. Exp. Med., 1991, 164 (3), 191-201 - A monoclonal antibody MRK16, recognizing specifically an epitope of P-glycoprotein (P-GP), a highly active efflux transporter of chemotherapeutic agents, was used to determine the degree of expression of P-GP in the normal human kidney and urinary bladder, and in kidney and urinary bladder cancers. P-glycoprotein was localized in the microvilli of the epithelial cells of the proximal renal tubules by immunoelectron microscopy, and detected immunohistochemiccally in 6 of 20 untreated kidney cancers and 11 of 31 untreated urinary bladder cancers. Some of the cancerous tissues were further examined with regard to P-GP expression by immunoprecipitation. In urinary bladder cancers, the degree of P-GP expression seemed to be somewhat correlated with tumor grading. These results indicate that our method to detect the degree of expression of P-GP by MRK16 may be applicable for the diagnosis of clinical multidrug resistant urinary cancers.

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