Immunogenicity of the outer domain of a HIV-1 clade C gp120
Open Access
- 17 May 2007
- journal article
- Published by Springer Nature in Retrovirology
- Vol. 4 (1) , 33
- https://doi.org/10.1186/1742-4690-4-33
Abstract
The possibility that a sub domain of a C clade HIV-1 gp120 could act as an effective immunogen was investigated. To do this, the outer domain (OD) of gp120CN54 was expressed and characterized in a construct marked by a re-introduced conformational epitope for MAb 2G12. The expressed sequence showed efficient epitope retention on the isolated ODCN54 suggesting authentic folding. To facilitate purification and subsequent immunogenicity ODCN54 was fused to the Fc domain of human IgG1. Mice were immunised with the resulting fusion proteins and also with gp120CN54-Fc and gp120 alone.Keywords
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