INTRACELLULAR-DISTRIBUTION AND RADIOTOXICITY OF CHROMIUM-51 IN MAMMALIAN-CELLS - AUGER-ELECTRON DOSIMETRY

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  • 1 January 1985
    • journal article
    • research article
    • Vol. 26  (1) , 59-67
Abstract
The kinetics of uptake and of radiotoxicity of 51Cr, an Auger-electron emitter, were studied in 51Cr Chinese hamster V79 lung fibroblasts. Intracellular radioactivity was directly proportional to the incubation period and to the extracellular concentration of 51Cr. About 14% of the cellular activity was associated with the nucleus; 2% was guanidine-precipitable and bound to DNA. The growth rate of V79 cells was slowed following intracellular incorporation of 51Cr. The cell-survival curve, in terms of colony-forming ability, was of the low-LET type, with a D37 [mean radioactive cellular content at 37% survival] of 6.2 pCi/cell. Theoretical dosimetric estimates indicated that, under the given experimental conditions, LD50 to the cell nucleus was 870 rad. Although this value was somewhat larger than the X-ray D37 dose of 580 rad for this cell line, was more realistic than the gross underestimate obtained by classical MIRD calculations (2-3 rad/cell).

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