A complex of single-strand binding protein and M13 DNA as hybridization probe
Open Access
- 1 January 1985
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 13 (8) , 2789-2802
- https://doi.org/10.1093/nar/13.8.2789
Abstract
Single-stranded DNA was complexed to the single-strand binding protein (SSB) of Escherichia coli in a mass ratio of 30:1. The protein moiety of this complex can be labelled by a number of methods of which we have chosen radio-iodination and biotinylation as examples. The SSB-M13 DNA complexes, labelled to high specific activities, were used as probes in hybridization experiments in which 1.6×10−18 moles of immobilized target DNA were detected. The stability of the hybrids was not severely decreased by the binding of SSB. Analysis of hybrids by electron microscopy showed that complexing of DNA with SSB could be used to allow its subsequent identification in the hybrids.Keywords
This publication has 21 references indexed in Scilit:
- Sandwich hybridization as a convenient method for the detection of nucleic acids in crude samplesPublished by Elsevier ,2003
- Interaction of single-strand binding protein and RecA protein at the single-stranded DNA siteJournal of Molecular Biology, 1983
- Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.Proceedings of the National Academy of Sciences, 1983
- Time-resolved fluorometer for lanthanide chelates--a new generation of nonisotopic immunoassays.Clinical Chemistry, 1983
- The making of strand-specific M13 probesGene, 1982
- Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.Proceedings of the National Academy of Sciences, 1981
- Sequences of the ssb gene and protein.Proceedings of the National Academy of Sciences, 1981
- Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedureNucleic Acids Research, 1979
- ELECTRON MICROSCOPE VISUALIZATION OF CHROMATIN AND OTHER DNA-PROTEIN COMPLEXESAnnual Review of Biophysics and Bioengineering, 1978
- Identification of specific crosslinked histones after treatment of chromatin with formaldehydeCell, 1975