A Rapid and Specific High-Performance Liquid Chromatographic Assay for Theophylline in Biological Fluids
- 1 June 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 28 (6) , 303-306
- https://doi.org/10.1093/chromsci/28.6.303
Abstract
A rapid, specific high-performance liquid chromatographic analysis of theophylline in plasma, serum, and saliva is described. Proteins present in the biological samples are precipitated with 6% perchloric acid and the clear supernatant is chromatographed on a reversed-phase column. Only 100 μL of serum is required and concentrations as low as 0.07 μ/mL can be measured accurately. Other xanthines do not interfere in the assay. Within- and betweenday variation is ⩽ 2.2%. The method shows less bias and greater precision than the TDx (Abbott Diagnostics) procedure commonly used in clinical laboratories.This publication has 3 references indexed in Scilit:
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