Die Abbildungseigenschaften autoradiographischer Systeme

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Abstract
In autoradiographic transfer two characteristic ranges can be distinguished which result from the composed kind of dose function (e−kR/R2). In “photographic region” of transfer, i. e. when thickness of emulsion and object are comparable with the maximum range of beta particles, the influence of absorption or beta energy is predominant and that of geometry can be neglected. The autoradiographic transfer properties are very similiar to those of photographic transfer. In the “crossfire region” of autoradiographic transfer i. e. when the thickness of emulsion and object are very small compared with the maximum particle range, the influence of geometric parameters is predominant. High crossfire causes blurred images, typical for microautoradiography with nuclides of high energies. In addition to linear transfer of periodic and nonperiodic distributions of radioactivity, the nonlinear transfer is considered. The influence of exposure on contrast transfer and grain density is also considered. Contrast measurements, made by using 131I- and 32P-labelled sine wave gratings, are in agreement with the calculated contrast transfer functions.

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