cis‐9,10‐Epoxystearic acid in human leukocytes: Isolation and quantitative determination
- 1 November 1988
- Vol. 23 (11) , 1015-1018
- https://doi.org/10.1007/bf02535645
Abstract
Racemiccis‐9,10‐epoxystearic acid was isolated from total lipids of human leukocytes. Identification of the epoxy acid was based mainly on gas liquid chromatographicmass spectrometric analysis and on its chemical conversion intothreo‐9,10‐dihydroxystearic acid. A mass spectrometric method for quantitative determination ofcis‐9,10‐epoxystearic acid using the tetradeuterated compound as internal standard was developed. Using this method, nonstimulated human leukocytes were found to contain 5.1±2.2 μg (SD) ofcis‐9,10‐epoxystearic acid per 109 cells (n=8). More than 90% of the epoxy acid occurred in its esterified form in leukocyte lipids.This publication has 18 references indexed in Scilit:
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