More Sensitive Automated Detection of Polyamines in Physiological Fluids and Tissue Extracts with o-Phthalaldehyde

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Abstract
O-Phthalaldehyde reacts with primary amines to form an intensely blue-fluorescing product. Replacing ninhydrin with this compound in our automated high-pressure liquid-chromatographic technique for determination of polyamines, we achieved a 6-to 10-fold increase in sensitivity. Linearity and reproducibility are excellent, and the method is shown to be applicable to analysis of physiological fluids and of tissue extracts.

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