NATURE OF ANTIGENIC COMPLEX RECOGNIZED BY T-LYMPHOCYTES .4. INHIBITION OF ANTIGEN-SPECIFIC T-CELL PROLIFERATION BY ANTIBODIES TO STIMULATOR MACROPHAGE IA ANTIGENS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 119  (1) , 223-226
Abstract
It was previously demonstrated that nonimmune guinea pig T [thymus-derived] lymphocytes could be specifically sensitized with TNP[trinitrophenol]-modified allogeneic macrophages after eliminating the alloreactive T cells with bromodeoxyuridine (BUdR) and light treatment. This procedure allowed the unique opportunity to use anti-Ia [immune response-associated antigen] sera directed against the Ia antigens of only the stimulator macrophages or responder T cells to determine against which cell type anti-Ia would block TNP-specific stimulation. The TNP-specific DNA synthetic response of BUdR and light-treated T cells stimulated with TNP-modified allogeneic macrophages was totally eliminated by anti-Ia sera directed solely against the allogeneic stimulator macrophage. Anti-Ia sera directed only against the responder T cells had no effect on their response to TNP-modified allogeneic macrophages. Macrophage Ia antigens are required for efficient T cell-macrophage interactions and raise the possibility that T cell Ia antigens may not be required for collaboration with macrophages. This latter possibility was substantiated by experiments in which it was shown that treating T cells with anti-Ia sera and complement to remove the Ia-positive cells before or after priming, or both, had no effect on their ability to be primed and restimulated with TNP-modified macrophages.

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