The flavohaemoglobin (HMP) of Escherichia coli generates superoxide in vitro and causes oxidative stress in vivo
- 11 March 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 382 (1-2) , 141-144
- https://doi.org/10.1016/0014-5793(96)00154-8
Abstract
Purified flavohaemoglobin (HMP) of Escherichia coli reduces Fe(III) in a superoxide dismuatase (SOD)-sensitive reaction, demonstrating superoxide anion generation during aerobic NADH oxidation. In vivo, sodA-lacZ fusion activity was increased 3-fold by introducing plasmid pPL341, containing the hmp gene, or by growth with paraquat. The effects were additive and SOXS-dependent. Thus HMP activity causes oxidative stress in vivo. Activities of sodA-lacZ and hmp-lacZ fusions were stimulated in a himA mutant, demonstrating repression of both promoters by integration host factor (IHF), but the effects of pPL341 on sodA-lacZ activity were not due to titration of IHF by the hmp promoter.Keywords
This publication has 24 references indexed in Scilit:
- O2-Sensing and O2-dependent gene regulation in facultatively anaerobic bacteriaArchiv für Mikrobiologie, 1995
- Binding of integration host factor (IHF) to the Escherichia coli sodA gene and its role in the regulation of a sodA-lacZ fusion geneMolecular Genetics and Genomics, 1995
- The dps promoter is activated by OxyR during growth and by IHF and σs in stationary phaseMolecular Microbiology, 1994
- Ferric Reductases in Escherichia coli: The Contribution of the Hemoglobin-like ProteinBiochemical and Biophysical Research Communications, 1994
- The oxygenated flavohaemoglobin from Escherichia coli: Evidence from photodissociation and rapid-scan studies for two kinetic and spectral formsBiochemical and Biophysical Research Communications, 1992
- The haemoglobin‐like protein (HMP) of Escherichia coli has ferrisiderophore reductase activity and its C‐terminal domain shares homology with ferredoxin NADP+ reductasesFEBS Letters, 1992
- Isolation and nucleotide sequence of the hmp gene that encodes a haemoglobin-like protein in Escherichia coli K-12Molecular Genetics and Genomics, 1991
- Improved single and multicopy lac-based cloning vectors for protein and operon fusionsGene, 1987
- Primary sequence of a dimeric bacterial haemoglobin from VitreoscillaNature, 1986
- Ferrozine---a new spectrophotometric reagent for ironAnalytical Chemistry, 1970