Experimental Studies on the Antigenicity of Some Metabolites and Related Compounds of Sulfanilamide

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Abstract
The antigenicity of some metabolites of sulfanilamide and their related compounds was studied by 3 kinds of experiments: protein binding, skin reaction in guinea pigs and antibody formation to 4-hydroxylaminobenzenesulfonamide (4-HABSA). The strong binding of 4-HABSA to bovine serum albumin (BSA) and rat serum protein was found by extensive equilibrium dialysis and gel filtration on a Sephadex G-25 column. In both groups of guinea pigs sensitized with serum albumin or 4-HABSA, positive skin reaction was observed 24 h after elicitation by injections of 4-HABSA, 4-nitro-sobenzenesulfonamide, 4,4''-azoxybenzenedisulfonamide, 4,4''-azobenzenedisulfonamide and 4,4''-hydrazobenzenedisulfonamide. 4-HABSA in the antisera of rabbits sensitized with 4-HABSA alone or 4-HABSA-BSA conjugate using passive hemagglutination tests were searched for. 4-HABSA conjugated with tissue proteins evidently acts as antigen, although the production of antibody to 4-HABSA hapten is low.

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