Dodecyl maltoside detergent improves resolution of hepatic membrane proteins in two‐dimensional gels
- 1 January 1991
- journal article
- Published by Wiley in Electrophoresis
- Vol. 12 (9) , 687-688
- https://doi.org/10.1002/elps.1150120919
Abstract
This report describes the incorporation of an alkyl maltoside detergent in two‐dimensional polyacrylamide gel Electrophoresis (2‐D PAGE) sample lysis buffer in order to improve resolution of protein patterns separated by nonequilibrium pH gradient Electrophoresis. Membrane‐associated proteins with alkaline isoelectric points form horizontal streaks on two‐dimensional electrophoretograms when solubilized with conventional nonionic detergent. Dodecyl maltoside enhances protein delipidation during solubilization and improves pattern resolution and protein mobility.Keywords
This publication has 9 references indexed in Scilit:
- Neutrophil-Specific Antigen NB1 Is Anchored Via a Glycosyl-Phosphatidylinositol LinkageJournal of Leukocyte Biology, 1991
- Chemical labeling studies on bovine heart mitochondrial cytochrome c oxidase dispersed in nonionic detergentsBiochemistry, 1990
- Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G‐250 and R‐250Electrophoresis, 1988
- Lipid and subunit III depleted cytochrome c oxidase purified by horse cytochrome c affinity chromatography in lauryl maltosideBiochemistry, 1983
- Analytical techniques for cell fractionsAnalytical Biochemistry, 1979
- Analytical techniques for cell fractionsAnalytical Biochemistry, 1978
- Analytical techniques for cell fractionsAnalytical Biochemistry, 1978
- High resolution two-dimensional electrophoresis of basic as well as acidic proteinsCell, 1977
- PREPARATION OF MITOCHONDRIA FROM ANIMAL TISSUES AND YEASTSPublished by Elsevier ,1972