Isolation of a Picorna-like Virus from Steelhead in Washington State
- 1 June 1992
- journal article
- Published by Oxford University Press (OUP) in Journal of Aquatic Animal Health
- Vol. 4 (2) , 90-96
- https://doi.org/10.1577/1548-8667(1992)004<0090:ioaplv>2.3.co;2
Abstract
In January 1990, a virus was isolated from ovarian fluid of steelhead Oncorhynchus mykiss from the Tokal Creek Hatchery in the southern portion of Puget Sound, Washington. A unique cytopathic effect, characterized by well‐defined plaques consisting of aggregates of multinucleate and vacuolated cells, was observed in five of seven cell lines inoculated with the virus. The virus grew best at 10 and 14°C, although it also replicated well at 6°C. It was resistant to incubation for 2 h at pH 3, 5, 7, and 9, but not at pH 2. The virus contained a single‐stranded RNA genome, and particles were often found as a lattice‐like matrix of unenveloped, round‐toicosahedral‐shaped nucleocapsids that were 25–30 nm in diameter and had a 16‐nm‐diameter core. Based on this information, we have classified the virus as the: Tokal Creek steelhead virus and believe it to be the first reported picorna‐like virus (possibly of the genus Enterovirus) of salmonids.Keywords
This publication has 0 references indexed in Scilit: