NOTES: Campylobacter jejuni subsp. doylei subsp. nov., a Subspecies of Nitrate-Negative Campylobacters Isolated from Human Clinical Specimens

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Abstract
We propose the name Campylobacter jejuni subsp. doylei for a new subspecies of gram-negative, microaerophilic Campylobacter, which is nitrate negative and was isolated from human clinical specimens (gastric epithelium biopsies and feces). The pathogenicity of the organism is not known. Strains grow poorly at 42.degree. C; most produce catalase, but some give weak or negative reactions; none reduces nitrate to nitrite. The guanine-plus-cytosine contents of the deoxyribonucleic acids (DNAs) of 10 strains were 29 .+-. 1 mol% (mean .+-. standard deviation). Conventional bacteriological tests and DNA-DNA hybridization experiments revealed that these strains form a tight phenotypic and genotypic cluster within C. jejuni. The characteristics which differentiate the new subspecies from other members of C. jejuni and from Campylobacter coli are given. The type strain is strain 093 (= NCTC 11951); it wa isolated from the feces of a young child with diarrhoea and has a guanine-plus-cytosine content of 29 mol%.