Comparative studies of the effects of cyclic AMP, various hormones and chloramphenicol on the induction of .DELTA.-aminolevulinic acid synthetase and tyrosine aminotransferase in the organ-cultured chick embryo liver.

Publisher Website
Google Scholar
Abstract
Induction of δ-aminolevulinic acid synthetase by allylisopropylacetamide in organ-cultured chick embryo liver was not appreciably influenced by any of cyclic AMP, dibutyryl cyclic AMP, theophylline, glucose, insulin, glucagon, epinephrine, isoproterenol, and hydrocortisone, whereas the activity of tyrosine aminotrans-ferase significantly increased in response to cyclic AMP and some of those hormones. Accumulation of δ-aminolevulinic acid synthetase in the cultured liver cytosol fraction was not appreciably increased by the addition of dibutyryl cyclic AMP or insulin to the incubation medium. Apparently the behaviors of the induction of δ-aminolevulinic acid synthetase in chick embryo liver in ovo and in vitro differ from those in the livers of adult chicken and rat. High concentrations of chloramphenicol suppressed significantly the allylisopropylacetamide-induced in-crease of 1-aminolevulinic acid synthetase as well as incorporation of 14C-leucine into proteins. The activity of tyrosine aminotransferase, however, was rather increased when relatively low concentrations of chloramphenicol were added to the medium.