Isolation of specific antigens fromangiostrongylus cantonensis 1. Preparative flatbed isoelectric focusing
- 1 January 1985
- journal article
- research article
- Published by Springer Nature in Zeitschrift Fur Parasitenkunde-Parasitology Research
- Vol. 71 (6) , 789-800
- https://doi.org/10.1007/bf00926804
Abstract
Electrophoresis on SDS gel and analytical isoelectric focusing showed that a crude extract ofAngiostrongylus cantonensis consisted of at least 40 protein components with molecular weights ranging from 13000–70000 and isoelectric points of pI values ranging from 3.7–10.0. Crossed-immunoelectrophoresis with a hyperimmune antiserum toA. cantonensis showed at least 40 different antigenic components in the crude worm extract which were cross-reactive with those ofAscaris suum, Metastrongylus apri andToxocara canis. Using preparative isoelectric focusing, the somatic worm preparation was divided into 13 equal fractions, of which 3, 4 and 5, with pI values of 3.7, 4.0 and 4.45 respectively, were later shown by immunoelectrophorectic techniques and enzymelinked immunosorbent assay to contain antigens specific toA. cantomensis.This publication has 12 references indexed in Scilit:
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