GROWTH CHARACTERISTICS OF INFLUENZA VIRUS

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Abstract
A further analysis of the growth curve obtained in vitro for influenza virus in chorioallantoic membrane was made using the viral inhibitor p-fluorophenylalanine. It was found that p-fluorophenylalanine is phase-specific, does not interfere with the initiation of infection but rather acts during the productive period of the infectious sequence. The site of action of this fluoro-derivative was described relative to the site of action of methoxinine. By the use of this inhibitor in combination with methoxinine, it was possible to recognize 2 stages of development which were not discernible from the usual growth curve. In a multicellular culture these 2 reactions, A and B, occur for the most part simultaneously, during the latent and productive periods. Reaction A is inhibited by methoxinine, but not by fluorophenylalanine. It begins early in the latent period and can proceed independent of reaction B. Reaction B is inhibited by fluorophenylalanine, but not by methoxinine, and it cannot proceed unless reaction A is proceeding or has operated for some prior interval.