Nanomolar Determination of the Ferrocene Derivatives Using a Recycling Enzyme Electrode. Development of a Redox Label Immunoassay
- 1 January 1995
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 28 (1) , 1-11
- https://doi.org/10.1080/00032719508006013
Abstract
A recycling bienzyme electrode for determination of nanomolar ferrocene concentration was developed. The electrode consists of a laccase – glucose dehydrogenase bienzyme membrane coupled with an oxygen electrode. Laccase catalyzes the oxidation of ferrocene with oxygen. Oxidized ferrocene is an electron acceptor for the reaction of glucose oxidation catalyzed by glucose dehydrogenase. The cyclic process of oxidation/reduction of ferrocene gives substrate recycling. The detection is achieved by measuring the decrease in oxygen concentration. In this system the detection limit for ferroceneacetic acid was 0.2 nM. Addition of anti-ferrocene-NCS antibodies blocked the electrode response to ferrocene-NCS, while they had no influence on the determination of ferrocenedicarbonic acid and paminophenol. The application of these electrodes to immunoassays with ferrocene derivatives as a label is discussed.Keywords
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