Specific Localization of β-ᴅ-Glucoside Conjugates of 2,4-Dichlorophenoxyacetic Acid in Soybean Vacuoles
Open Access
- 1 September 1982
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 37 (9) , 772-777
- https://doi.org/10.1515/znc-1982-0907
Abstract
Cell suspension cultures of soybean (Glycine max L.) were incubated with [14C]-2,4- dichlorophenoxyacetic acid (62 h, 27 °C). The cells were converted to protoplasts by use of the cell-wall degrading enzymes, Driselase and pectinase. This procedure resulted in the release of the cellular amino acid conjugates as well as part of the free 2,4-dichlorophenoxyacetic acid and of the β-ᴅ- glucoside conjugates. A vacuole fraction was obtained by mild osmotic shock in 10% yield, and was characterized by its polypeptide composition. The purified vacuoles were enriched in β-ᴅ-glucoside conjugates, free 2,4-dichlorophenoxyacetic acid being only a minor vacuolar constituent, and amino acid conjugates not being detectable.Keywords
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