Combined Use of Released Proteins and Lipopolysaccharide in Enzyme-Linked Immunosorbent Assay for Serologic Screening of Yersinia Infections
Open Access
- 1 February 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 163 (2) , 409-412
- https://doi.org/10.1093/infdis/163.2.409
Abstract
An ELISA for the screening of serum antibodies to Yersinia species wasdeveloped using plasmidencoded released proteins of Yersinia enterocolitica 0:8 and lipopolysaccharide of 1': enterocolitica0: 3 as a combined antigen. Of 43 sera from patients infected with one of six different Yersinia serotypes, 40 (93%) were positive in this assay. When tested using six serotype-specific ELISAs with the corresponding Yersinia bacteria as antigens, 38 (88%) were positive. Thisscreening ELISA detects antibodies to all virulent yersiniae in one assay and offers the possibility for diagnosis of infections caused by Yersinia serotypes seen only occasionally and not usually included in the serotype-specific ELISAs. Thus, this ELISA offers a substantial advantage by saving time and money in routine laboratory work.Keywords
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