A model to explain the osmotic pressure behavior of hemoglobin and serum albumin
- 1 May 1990
- journal article
- research article
- Published by Canadian Science Publishing in Biochemistry and Cell Biology
- Vol. 68 (5) , 894-898
- https://doi.org/10.1139/o90-132
Abstract
Previously published osmotic pressure data on hemoglobin and bovine serum albumin were used to determine the osmotically unresponsive solvent volume per unit dry mass of protein. A model is presented that accounts for the osmotic pressure of globular proteins based on a surface-associated osmotically unresponsive solvent volume. The model also accounts for changes in the osmotically unresponsive solvent volume owing to changes in pH, cosolute salt concentration, protein conformation, and protein aggregation.Key words: hemoglobin, serum albumin, osmotic pressure, water of hydration, salt, pH, model of protein structure.This publication has 4 references indexed in Scilit:
- Water of hydration in the intra- and extra-cellular environment of human erythrocytesBiochemistry and Cell Biology, 1988
- Chemical potential measurements of deoxyhemoglobin S polymerizationJournal of Molecular Biology, 1985
- Analysis of non-ideal behavior in concentrated hemoglobin solutionsJournal of Molecular Biology, 1977
- A theory of partial osmotic pressures and membrane equilibria, with special reference to the application of Dalton's Law to hæmoglobin solutions in the presence of saltsProceedings of the Royal Society of London. Series A, Containing Papers of a Mathematical and Physical Character, 1928