The developmental changes of mRNA levels for a cerebellar protein (spot 35 protein) in rat brains

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Abstract
Our previous papers described a protein called spot 35 found in the cerebellar cytosol of adult rats by two‐dimensional gel electrophoresis and localized in the Purkinje cells by immunohistochemical methods. Here we describe the biosynthesis of this spot 35 protein using a reticulocyte lysate cell‐free system containing rat cerebellar mRNA. The developmental changes of mRNA‐dependent protein biosynthesis were also examined. During postnatal 10–30 days, a rapid increase of mRNA levels for spot 35 protein was observed. The application of the new 45Ca‐binding assay procedure revealed that this protein is a Ca‐binding protein.