POTASSIUM-PERMANGANATE REACTION IN AMYLOIDOSIS - HISTOLOGIC METHOD TO ASSIST IN DIFFERENTIATING FORMS OF THIS DISEASE

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 36  (3) , 274-281
Abstract
The method is based on the affinity of amyloid for Congo red dye after exposure to potassium permanganate and dilute sulfuric acid. The permanganate method represents a modification of the Romhanyi trypsin technique. The permanganate reaction was applied to a series of amyloid samples of known amino acid composition, to amyloid samples fixed in a variety of different preservatives and to tissues obtained at autopsy from 67 amyloidosis patients whose disease was previously subclassified on the basis of clinical presentation and autopsy observations. This method distinguished amyloid protein amino acids from other varieties of amyloid and proved effective when applied to amyloid samples preserved in any of several commonly used fixatives. This simple histochemical method proved useful in subclassifying amyloid type in the patient series particularly when used in conjunction with the available clinical history and the organ distribution of amyloid accumulation.

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