Accumulation of exoglucanase activity in yeast secretory mutants blocked at the endoplasmic reticulum level
- 17 February 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 196 (2) , 291-295
- https://doi.org/10.1016/0014-5793(86)80265-4
Abstract
Saccharomyces cerevisiae HMSF‐176 (sec 18), a thermosensitive secretory mutant blocked at the endoplasmic reticulum (ER) level, drastically increased its osmotic sensitivity when grown at the restrictive temperature of 37°C in high glucose concentration. This fact led to the erroneous interpretation that glucanases were inactive when localized in the ER. The development of a suitable osmotic stabilizer now indicates that sec 18 accumulates exoglucanase activity. Another ER‐blocked mutant behaved in a similar way. All the accumulated exoglucanase was found in a soluble form. By contrast, a significant portion of the accumulated invertase remained in a membrane‐bound form.Keywords
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