Structural characterization of the N-linked oligosaccharides in bile salt-stimulated lipase originated from human breast milk
Open Access
- 1 March 1999
- journal article
- research article
- Published by Oxford University Press (OUP) in Glycobiology
- Vol. 9 (3) , 227-234
- https://doi.org/10.1093/glycob/9.3.227
Abstract
The detailed structures of N-glycans derived from bile saltstimulated lipase (BSSL) found in human milk were determined by combining exoglycosidase digestion with matrixassisted laser desorption/ionization time-of-flight mass spectrometry. The N-glycan structures were conclusively determined in terms of complexity and degree of fucosylation. Ion-exchange chromatography with pulsed amperometric detection, together with mass-spectral analysis of the esterified N-glycans, indicated the presence of monosialylated structures. The molecular mass profile of esterified N-glycans present in BSSL further permitted the more detailed studies through collision-induced dissociation (CID) and sequential exoglycosidase cleavages. The N-glycan structures were elucidated to be complex/dibranched, fucosylated/complex/dibranched, monosialylated/complex/dibranched, and monosialylated/fucosylated/dibranched entities.Keywords
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