Results of a Cooperative Study Comparing the Precision of Peak Height and Area Measurements in Liquid Chromatography

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Abstract
This report presents results from a cooperative study involving 50 laboratories assessing the practical precision of liquid chromatographic analyses. Specifically, the precision of quantitation by peak area was compared with that by peak height for two four-component solutions. Results from this study indicate that the method of choice for quantitation depends on the chromatographic behavior of individual peaks. For well-behaved chromatographic peaks, the precision of area measurements was equal to or better than that of height measurements. However, in cases where peaks were poorly resolved from the solvent front and attendant baseline perturbations, or where a peak eluted on the tail of an earlier eluting peak, peak height measurements appeared to provide more precise quantitation. These studies were carried out with isocratic elution; comparisons under gradient elution conditions were not made. This study was conducted by ASTM Subcommittee E-19.08.

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