Determination of Identity in Paraffin-Embedded Tissues and Slides

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Abstract
A procedure is presented for the determination of donor identity or commonality of origin among paraffinembedded tissues and slides. Samples are de-paraffinized with xylene and ethanol and subjected to ChelexR extraction. The Polymarker forensic DNA typing kit is utilized for identity testing. Following multiplex amplification, PCR product is verified on an agarose gel and then the samples are geiotyped using reverse dot-blot hybridization. When this procedure was applied to paraffin-embedded samples involved in a legal action, all samples were successfully genotyped. The advantages of using this procedure are discussed.