Colorimetric Assay of Reserpine in Formulations and Biological Fluids
- 1 July 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 59 (4) , 795-798
- https://doi.org/10.1093/jaoac/59.4.795
Abstract
Two colorimetric methods are presented for determining reserpine. In the first method, an iron hydroxamate complex is formed through the ester group in position 16 in the reserpine molecule. The color is measured at 535 nm (0.5–6 mg/25 ml). This method is useful for routine and control analyses of reserpine formulations. In the second method the tertiary amino group of reserpine reacts with 2% citric acid in acetic anhydride to form a red-violet complex which is measured at 505 nm (5–400 μg/10 ml). This method could be useful in measuring trace amounts of reserpine present in biological fluids.This publication has 5 references indexed in Scilit:
- Assay Methods for Some Vinca rosea Alkaloids IIJournal of Pharmaceutical Sciences, 1964
- A Simplified Nitrite Procedure for the Determination of Reserpine**Division of Pharmaceutical Chemistry, Bureau of Biological and Physical Sciences, Food and Drug Administration, Department of Health, Education, and Welfare, Washington, D. C.Journal of the American Pharmaceutical Association (Scientific ed.), 1957
- STUDIES ON THE METABOLISM OF RESERPINE1956
- The Determination of Reserpine by Chromatographic Separation from Pharmaceutical ProductsJournal of the American Pharmaceutical Association (Scientific ed.), 1955
- Determination of Reserpine in Pharmaceutical Preparations*Journal of the American Pharmaceutical Association (Scientific ed.), 1955