The genes coding for tRNATyr of Drosophila melanogaster: Localization and determination of the gene numbers
- 1 September 1981
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 84 (1) , 49-60
- https://doi.org/10.1007/bf00293362
Abstract
Transfer RNATyr (anticodon GΨA) was isolated from Drosophila melanogaster by means of Sepharose 4B, RPC-5, and polyacrylamide gel electrophoresis. The tRNA was iodinated in vitro with Na125I and hybridized in situ to salivary gland chromosomes from Drosophila. The genes of tRNATyr were localized in eight regions of the genome by autoradiography. Restriction enzyme analysis of genomic DNA indicated that the haploid Drosophila genome codes for about 23 tRNATyr genes. The regions 22F and 85A each contain four to five tRNATyr genes, whereas the regions 28C, 41AB, 42A, 42E, and 56D each contain two to three tRNATyr genes.Keywords
This publication has 43 references indexed in Scilit:
- The sequence of the 3′ non-coding region of the hexon mRNA discloses a novel adenovirus geneNucleic Acids Research, 1981
- In situ hybridization of three transfer RNAs to the polytene chromosomes of Drosophila melanogasterJournal of Molecular Biology, 1980
- Two control regions for eukaryotic tRNA gene transcription.Proceedings of the National Academy of Sciences, 1980
- The actin genes of drosophila: a dispersed multigene familyCell, 1980
- Collection of mutant tRNA sequences.Nucleic Acids Research, 1980
- Transfer RNA genes of Drosophila melanogasterNucleic Acids Research, 1980
- Nonsense suppression in eukaryotes: the use of the Xenopus oocyte as an in vivo assay systemNucleic Acids Research, 1980
- The Localization of Transfer RNA 5 Lys Genes in Drosophila melanogasterProceedings of the National Academy of Sciences, 1974
- Characterization of precursors to tRNA in yeastFEBS Letters, 1973
- FORMATION AND DETECTION OF RNA-DNA HYBRID MOLECULES IN CYTOLOGICAL PREPARATIONSProceedings of the National Academy of Sciences, 1969