HPLC Analysis of Diuron and Metabolites in Blood and Urine

Abstract
A high-pressure liquid chromatographic method for the determination of diuron and its metabolites in human urine and blood is presented. The synthesis of different metabolites and of a suitable internal standard is described and the structure of the compounds is determined by mass spectrometry and nuclear magnetic resonance spectroscopy. The method is applied to an overdose case.

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