CD23 expression on B‐lymphocytes and its modulation by cytokines in allergic patients

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Abstract
Summary: The aim of this study was to assess the expression of CD23 on peripheral blood B‐cells. and its in vitro modulation by recombinant human interferon‐γ (IFN‐γ) in phytohaem‐agglutinin‐ (PHA) or recombinant human interleukin‐4 (IL‐4)‐stimulaled cultures in atopic patients with Dermatophagoides pteromyssinus hyprrsensitivity and in healthy non‐atopic subjects. Atopic patients with asthma not receiving allergen‐specific immunotherapy (n= 21) were studied and further compared with a group of atopic subjects with asthma under allergen‐specific immunotherapy (n = 21). They were age‐(± 5 yr) and sex‐matched. The results were also compared with those obtained in the non‐atopic group (n= 11).CD23 expression on B‐lymphocytes and its modulation were analysed by flow cytometry using conjugated monoclonal antibodies with a double immunofluorescence method. Atopic patients had an increase in the percentage of B‐cells expressing CD23 in peripheral blood. Phytohaemagglutinin and IL‐4 induced a rise in the percentage of CD23‐positive B‐cells in both atopic groups and non‐atopic subjects. Phytohaemagglutinin provoked an increase in the intensity of CD23 expression on B‐cells from stimulated cultures in all groups, while IL‐4 only produced a significant increase in atopic patients. The presence of IFN‐γ decreased the CD23 expression on B‐cells in PH A‐stimulated culture of atopic patients, whereas it caused an increase in CD23 expression in the non‐atopic group. Furthermore, the presence of IFN‐γ in IL‐4‐stimulatcd cultures induced a decrease in CD23 expression on B‐cells in all cases. These results indicate a difference between atopic and non‐alopic donors in their responses to IL‐4 and IFN‐γ. On the other hand, allergen‐specific immunotherapy did not induce any shift in the expression and modulation of this receptor.

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