The determination of phylloerythrin in blood

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Abstract
Details are given of a method for estimating phylloerythrin in blood, consisting of extraction into ether-acetic acid solution, distribution between ether and HC1 solutions, and spectrophotometric measurement. The inflexion at 4260 A in the absorption spectrum of phylloerythrin provides a quantitative check on the purity of the material being measured. The method is applicable over the range 0.05-3.5 mg of phylloerythrin/100 ml of plasma, but a modification giving approximate values down to 0.005 mg/ 100 ml is described. Protoporphyrin and other porphyrins do not cause significant interference.