Growth of Dispira Cornuta in Artificial Culture

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Abstract
SUMMARY(1) Dispira cornuta was isolated and cultivated on certan artificial media. The methods of isolation and culture are given, also the extent of growth and of sporulation on various media is tabulated. TABLE 1 Growth of Dispira Cornuta in Artificial Culture(2) Nutrient agar media were found to be unsuitable for the cultivation of D. cornuta; but on solid media as swordfish, meat and egg yolk, which are rich in certain proteins, it grows and sporulates satisfactorily.(3) Liquid media containing only proteins, supported vegetative growth but not sporulation.(4) Carbohydrates and fats either in liquid or solid media were unsuitable for the growth of the fungus.(5) The optimum temperature for growth and sporulation and also for the germination of the spores is between 21° and 27° C., the minimum is near 8°. C.; and the maximum temperature is about 35° C.(6) D. cornuta, although it has been cultured for several years on various artificial media is still able to parasitize the same hosts as when first isol...

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