Bioassay-Directed Fractionation of the Organic Extract of SRM 1649 Urban Air Particulate Matter
- 1 July 1990
- journal article
- research article
- Published by Taylor & Francis in International Journal of Environmental Analytical Chemistry
- Vol. 39 (3) , 245-256
- https://doi.org/10.1080/03067319008032068
Abstract
Separation of 2 grams of the methylene chloride extract of NIST SRM 1649 (Washington, D.C. urban air particulate matter) into six compound class fractions by acid-base partitioning and silica gel column chromatography is demonstrated here. Recoveries of organic mass and Salmonella typhimurium TA98 mutagenic activity were greater than 80%. The fractionation method showed reproducible distribution of both mass and mutagenic activity; the compound class mass distribution results were similar to those obtained for more typical analytical fractionation of a milligram quantity of the extract mass. The most polar compound class fractions contained the greatest proportions of mutagenic activity.Keywords
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