A specific Goheterotrimer couples somatostatin receptors to voltage-gated calcium channels in RINm5F cells
Open Access
- 12 February 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 380 (1-2) , 137-141
- https://doi.org/10.1016/0014-5793(96)00047-6
Abstract
The peptide hormone somatostatin inhibits glucose‐induced insulin secretion in the rat insulinoma RINm5F cells by inhibition of voltage‐gated calcium channels. Here we used microinjection of antisene oligonucleotides directed against subtypes of G‐protein subunits to determine the subunit composition involved in somatostatin‐induced inhibition of voltage‐gated calcium channels in RINmF5 cells. Injection of antisene oligonucleotides annealing to the respective mRNA of Gαo2, Gβ1 and Gγ3 reduced the somatostatin‐induced inhibition of calcium channels in these cells. Injection of antisene oligonucleotides directed against other G‐protein subunits did not, suggesting that in RINm5F cells the somatostatin receptor couples to a G protein of Gαo2β1γ3 composition. By using a selective agonist of type 2 somatostatin receptors (SSTR 2) NC 8–12, we identified this receptor as the subtype coupling to calcium channels in RINm5F cells.Keywords
This publication has 13 references indexed in Scilit:
- Classification and nomenclature of somatostatin receptorsTrends in Pharmacological Sciences, 1995
- Somatostatin receptor activation of cellular effector systemsCellular Signalling, 1995
- Molecular pharmacology of somatostatin receptorsNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1994
- Selectivity in Signal Transduction Determined by γ Subunits of Heterotrimeric G ProteinsScience, 1993
- Molecular biology of somatostatin receptorsTrends in Neurosciences, 1993
- The Somatostatin Receptor in the GI TractAnnual Review of Physiology, 1992
- Different β-subunits determine G-protein interaction with transmembrane receptorsNature, 1992
- Biochemical and Functional Properties of Somatostatin ReceptorsJournal of Neurochemistry, 1992
- Assignment of G-protein subtypes to specific receptors inducing inhibition of calcium currentsNature, 1991
- 3H-1,2-Benzodithiole-3-one 1,1-dioxide as an improved sulfurizing reagent in the solid-phase synthesis of oligodeoxyribonucleoside phosphorothioatesJournal of the American Chemical Society, 1990