EFFECT OF MUTATIONS IN SURFACE LIPOPOLYSACCHARIDE OR OUTER MEMBRANE PROTEIN OF ESCHERICHIA COLI C ON TRANSFECTING COMPETENCE FOR MICROVIRID PHAGE DNA

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Abstract
Ca2+- or Ba2+-dependent uptake of microvirid phage DNA was not affected by the presence or absence of a phage receptor on the cell surface of E. coli C. Structural changes of cell wall lipopolysaccharide in E. coli C affected the development of Ca2+- or Ba2+-dependent competence. Efficiency of transfection was low in strain C61 (lacking branch heptose) and in C23.cntdot.1 (deficient in galactose residues and branch glucose). Ba2+-induced competence was also low in the terminal galactose-less strain C23. Deficiency in murein lipoprotein resulted in an increased competence for single-stranded DNA and double-stranded replicative form DNA. Level of the competence of mutants resistant to T5 and/or T1 was not much different from that of the sensitive bacteria. In various strains of E. coli, Ca2+(Ba2+)-induced competence for single-stranded DNA was parallel with that for double-stranded replicative form DNA. The ratio of Ca2+- and the Ba2+-dependent competence varied considerably in several derivatives of strain C.

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