Effects of Antineoplastic Drugs on Plateau-Phase Cultures of Mammalian Cells. I. Description of the Plateau-Phase System2

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Abstract
The plateau phase of growth was studied in 2 mammalian cell lines, each cultured in 2 different media. The onset of the plateau phase appeared to depend on the medium and the percent of serum used in the cell cultures. The analysis of kinetic parameters demonstrated that the fraction of cells which proliferated during the plateau phase varied in the 2 lines and for the different culture conditions, but was rarely near zero. Further experiments on a Chinese hamster cell line cultured in MEM supplemented with 15% fetal calf serum permitted us to distinguish several population compartments containing proliferating or non proliferating cells and clonigenic or nonclonigenic cells and to outline some of the relations between compartments. In particular, clonigenic cells (monitored by trypsinization, dilution, reseeding, and colony formation) seemed to include only cells that were not synthesizing DNA at the time of trypsinization. The overall kinetic patterns of the plateau-phase population resembled those of cell-renewal systems in vivo. These results are important for understanding lethal and kinetic effects of antineoplastic drugs administered to plateauphase cultures.

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