INCORPORATION OF [1‐14C]OLEIC ACID AND [1‐14C]ARACHIDONIC ACID INTO LIPIDS IN THE SUBCELLULAR FRACTIONS OF MOUSE BRAIN

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Abstract
Abstract— The distribution of radioactivity among lipids of subcellular membrane fractions was examined after intracerebral injections of [1‐14C]oleic and [1‐14C]arachidonic acids. Labelled free fatty acids were distributed among the synaptosomal‐rich, microsomal, myelin and cytosol fractions at 1 min after injection. However, incorporation of the fatty acids into phospholipids and trïacylglycerols after pulse labelling occurred mainly in the microsomal and synaptosomal‐rich fractions. With both types of labelled precursors, there was a higher percentage of radioactivity of diacyl‐glycerophosphoryl‐inositols in the synaptosomal‐rich fraction as compared to the microsomal fraction. Radioactivity of [1‐14C]oleic acid was effectively incorporated into the triacylglycerols in the microsomal fraction whereas radioactivity of the [1‐14C]arachidonic acid was preferentially incorporated into the diacyl‐glycerophosphorylinositols in the synaptosomal‐rich fraction. Result of the study indicates that synaptosomal‐rich fraction in brain is able to metabolize long chain free fatty acids in vivo and to incorporate these precursors into the membrane phosphoglycerides.

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