Volatile Preservatives for Culture Media

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Abstract
A mixture by volume of 1 part o-fluorotoluene, 2 parts n-butyl chloride, and 1 part 1,2-dichlorethane, is recommended as a chemically nearly inert but nevertheless effective preservative for biological solutions. This preservative, used at approximately the 1% level, is completely removed under the usual conditions of steam sterilization.

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