Participation of the tRNA A76 Hydroxyl Groups throughout Translation
- 25 April 2006
- journal article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 45 (19) , 5939-5948
- https://doi.org/10.1021/bi060183n
Abstract
The free 2‘−3‘ cis-diol at the 3‘-terminus of tRNA provides a unique juxtaposition of functional groups that play critical roles during protein synthesis. The translation process involves universally conserved chemistry at almost every stage of this multistep procedure, and the 2‘- and 3‘-OHs are in the immediate vicinity of chemistry at each step. The cis-diol contribution affects steps ranging from tRNA aminoacylation to peptide bond formation. The contributions have been studied in assays related to translation over a period that spans at least three decades. In this review, we follow the 2‘- and 3‘-OHs through the steps of translation and examine the involvement of these critical functional groups.Keywords
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