Cloning and Expression of Class A β-Lactamase Gene blaA BPS in Burkholderia pseudomallei
Open Access
- 1 April 2002
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 46 (4) , 1132-1135
- https://doi.org/10.1128/aac.46.4.1132-1135.2002
Abstract
The β-lactamase gene blaA BPS in Burkholderia pseudomallei was cloned and expressed in Escherichia coli . BPS-1 is a cephalosporinase with an isoelectric point of 7.7. Sequence analysis of BPS-1 revealed conserved motifs typical of class A β-lactamases and a relationship to the PenA (in B. cepacia ) and BlaI (in Yersinia enterocolitica ) lineages.Keywords
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