QUANTITATIVE LOCAL CEREBRAL BLOOD-FLOW MEASUREMENTS WITH TECHNETIUM-99M HM-PAO - EVALUATION USING MULTIPLE RADIONUCLIDE DIGITAL QUANTITATIVE AUTORADIOGRAPHY

  • 1 August 1988
    • journal article
    • research article
    • Vol. 29  (8) , 1387-1392
Abstract
We investigated d,1 [99mTc]hexamethylpropyleneamine oxime complex (HM-POA) as a tracer for quantitative measurement of local cerebral blood flow (LCBF) in a series of awake male rats. LCBF measurements with HM-PAO were compared to those of two other tracers, [14C]iodoantipyrine (IAP) and [201Tl]diethyldithiocarbamate (DDC), using quantitative double and triple tracer digital autoradiography. LCBF values with HM-PAO averaged 64% those of IAP and were generally linearly related. Detailed analysis suggested that the underestimation of LCBF by HM-PAO was related to blood constituent binding and/or rapid conversion to a noncerebrophilic compound, as well as noninstantaneous cerebral trapping, rather than to diffusion limitation.