Stereochemical Analysis of the Enzymic Synthesis and Hydrolysis of Ap4A
- 1 January 1991
- journal article
- plenary lectures
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 10 (1-3) , 181-193
- https://doi.org/10.1080/07328319108046445
Abstract
The stereochemical course of synthesis of PlP4-Bis(5′-adenosyl) tetraphosphate (Ap4A) from ATP catalysed by phenylalanyl-tRNA synthetase in the presence of Zn2+ has been shown to proceed with retention of configuration at Pα in accord with the mechanism involving phenylalanyl-adenylate as an intermediate. The hydrolysis of Ap4A by the unsymmetrical Ap4A phosphodiesterase from lupin seeds proceeds with inversion of configuration at Pα indicating that the hydrolysis takes place by a direct 'in line' displacement mechanism.Keywords
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