Cloning and characterization of an Escherichia coli gene, pcnB, affecting plasmid copy number
- 1 July 1989
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 3 (7) , 903-910
- https://doi.org/10.1111/j.1365-2958.1989.tb00239.x
Abstract
A gene, pcnB, affecting the copy number of ColE1-related plasmids has been cloned and mapped to 3.6 min on the Escherichia coli chromosome between panD and fhu. The gene encodes a previously undescribed 48 kD protein. Several independently isolated mutants exhibiting the same phenotype, reduced copy number, have been shown to be pcnB-.This publication has 26 references indexed in Scilit:
- Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetiiCellular Microbiology, 2007
- The physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic libraryCell, 1987
- Control of cole 1 plasmid replication: The process of binding of RNA I to the primer transcriptCell, 1984
- Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replicationGene, 1983
- TECHNOLOGICAL EXAMINATION OF LOW‐FIRED TERRACOTTA STATUES FROM AYIA IRINI, KEAArchaeometry, 1982
- Construction and characterization of new cloning vehicles IV. Deletion derivatives of pBR322 and pBR325Gene, 1980
- Trans-complementable copy-number mutants of plasmid ColE1Nature, 1980
- Construction and characterization of new cloning vehicles III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA moleculesGene, 1978
- R plasmid gene dosage effects in Escherichia coli K-12: Copy mutants of the R plasmid R1drd-19Plasmid, 1977
- A preliminary map of genomic sites for F-attachment inBiochemical and Biophysical Research Communications, 1964